Human Pro-Opiomelanocortin

Mfr.No.

E3425Hu

Sensitivity

14.84ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Pro-opiomelanocortin (also known as POMC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ACTH;Adrenocorticotropic hormone;Alpha-MSH;Beta-endorphin;Beta-LPH;Beta-MSH;CLIP;Corticotropin;Corticotropin-like intermediary Peptide;Corticotropin-lipotropin;Gamma-LPH;Gamma-MSH;Lipotropin beta;Lipotropin gamma;Melanocyte-stimulating hormone alpha;Melanocyte-stimulating hormone beta;Melanotropin alpha;Melanotropin beta;Melanotropin gamma;Met-enkephalin;NPP;POMC;Potential Peptide;Pro-opiomelanocortin

Molecular Weight

29424

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3425Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2695.3	80.41	3
Intra-Assay	2	18	1550.3	89.56	5.8
Intra-Assay	3	18	958.3	56.99	5.9

Background

(Corticotropin): Stimulates the adrenal glands to release cortisol. (Melanocyte-stimulating hormone alpha): Anorexigenic Peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. (Melanocyte-stimulating hormone beta): Increases the pigmentation of skin by increasing melanin production in melanocytes. (Beta-endorphin): Endogenous orexigenic opiate. (Met-enkephalin): Endogenous opiate.

Protocol

These standard curves of E3425Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.423	2.459	2.409
	2.494
2400ng/L	1.43	1.411	1.361
	1.392
1200ng/L	0.749	0.78	0.73
	0.811
600ng/L	0.398	0.4	0.35
	0.402
300ng/L	0.233	0.243	0.193
	0.253
0ng/L	0.06	0.05	0
	0.04

Gene ID	5443
UniProt ID	P01189

Research Area	Cancer

Storage	2-8°C

Reference	Association between Ag-RP, alpha-MSH and cardiovascular risk factors regarding adherence to diet quality index-international (DQI-I) among obese individuals Journal: J Cardiovasc Thorac Res. 2021;13(4):320-329 Hypothalamic-pituitary hormones will be affected by the interaction between 5q13-14-rs2239670 (CARTPT) gene variants and diet in different obesity phenotypes Journal: BMC Res Notes. 2021 Dec 7;14(1):443 Cocaine and amphetamine-regulated transcript prepropeptide gene (CARTPT) polymorphism interacts with Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI) to affect hypothalamic hormones and cardio-metabolic risk factors among obese individuals Journal: J Transl Med. 2020 Jan 9;18(1):16

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104952	E3425Hu	48T	$320		Add to Cart
BHE12104952	E3425Hu	96T	$458		Add to Cart

Human Pro-Opiomelanocortin - POMC ELISA Kit

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