Human Pro-Cathepsin B ELISA Kit PicoKine®

Mfr.No.

EK2130

Sensitivity

<20 pg/ml

Detection Range

0.156 ng/ml - 10 ng/ml

Reactivity

Human

Sample Volume

Concentration(ng/ml)	0	0.156	0.312	0.625	1.25	2.5	5	10
O.D.	0.019	0.138	0.241	0.334	0.409	0.719	1.304	1.902

Description

Human Pro-Cathepsin B ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSB in cell culture supeatants, serum, plasma (heparin), saliva and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CTSB; CPSB; Cathepsin B; EC 3.4.22.1; APP secretase; APPS; Cathepsin B1 (Cleaved into: Cathepsin B light chain; Cathepsin B heavy chain)

Immunogen

Expression system for standard: NS0; Immunogen sequence: R18-I339

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CTSB

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	225	1024	1858	247	1103	1963
Standard deviation	13.28	53.25	91.04	17.54	59.56	133.48
CV(%)	5.9%	5.2%	4.9%	7.1%	5.4%	6.8%

Background

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	225	269	241	216	238	8.08	3.4%
Sample 2	1024	1103	985	1240	1088	26.11	2.4%
Sample 3	1858	1956	1785	2006	1901	58.94	3.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1508
Gene Name	CTSB
UniProt ID	P07858
Protein Name	Cathepsin B

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001676	EK2130	96 wells/kit	$499		Add to Cart

Human Pro-Cathepsin B ELISA Kit PicoKine®

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