Human Potassium - Sodium Hyperpolarization-activated Cyclic Nucleotide-gated Channel 4

Mfr.No.

E4849Hu

Sensitivity

4.05ng/L

Detection Range

8-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Potassium,Sodium Hyperpolarization-activated Cyclic Nucleotide-gated Channel 4 (also known as HCN4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BRGDA8;EIG18;HCN4;Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated Channel 4;SSS2

Molecular Weight

129042

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4849Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	334.57	19.03	5.69
Intra-Assay	2	18	885.47	29.41	3.32
Intra-Assay	3	18	160.28	9.93	6.2

Background

Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih). May mediate responses to sour stimuli.

Protocol

These standard curves of E4849Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.297	2.298	2.254
	2.298
600ng/L	1.322	1.34	1.297
	1.358
300ng/L	0.938	0.858	0.814
	0.778
150ng/L	0.471	0.449	0.405
	0.426
75ng/L	0.238	0.248	0.205
	0.259
0ng/L	0.048	0.044	0
	0.039

Gene ID	10021
UniProt ID	Q9Y3Q4

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106124	E4849Hu	48T	$320		Add to Cart
BHE12106124	E4849Hu	96T	$458		Add to Cart

Human Potassium - Sodium Hyperpolarization-activated Cyclic Nucleotide-gated Channel 4 - HCN4 ELISA Kit

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