Human Polyamine-Modulated Factor 1-binding Protein 1

Mfr.No.

E3187Hu

Alternative Names

PMF-1-binding protein;PMFBP 1;PMFBP1;Polyamine-modulated factor 1-binding protein 1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

117494

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.58ng/L

Background

Required for normal spermatogenesis (PubMed: 1770140, PubMed: 30032984, PubMed: 30298696). It functions as a scaffold protein that attaches the sperm head-tail connecting piece to the nuclear envelope, thus maintaining sperm head and tail integrity (PubMed: 30032984). May also be involved in the general organization of cellular cytoskeleton (By similarity).

Gene ID	83449
UniProt ID	Q8TBY8
Antigen	PMFBP1

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E3187Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.073	2.103	2.054
	2.133
1200ng/L	1.613	1.645	1.596
	1.677
600ng/L	1.008	0.989	0.94
	0.971
300ng/L	0.784	0.777	0.728
	0.771
150ng/L	0.42	0.413	0.363
	0.405
0ng/L	0.052	0.049	0
	0.047

Precision

We measured random samples of E3187Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	177.1	11.75	6.6
Intra-Assay	2	18	573.3	43.72	7.6
Intra-Assay	3	18	813.6	41.4	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104716	E3187Hu	48T	$320		Add to Cart
BHE12104716	E3187Hu	96T	$458		Add to Cart

Human Polyamine-Modulated Factor 1-binding Protein 1 - PMFBP1 ELISA Kit

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