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Home / ELISA Kit

Human Poly ADP ribose polymerase (PARP) ELISA Kit

SKU : BHE13703518

PARP is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress . This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo . In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) .
PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.

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Mfr.No.	KTE61302
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Poly ADP ribose polymerase (PARP) ELISA Kit employs a two-site sandwich ELISA to quantitate PARP1.
Alternative Names	PARP1; RP11-125A15.2; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); poly (ADP-ribose) polymerase family; member 1; po
Detection Method	Colorimetric
Kit Components	Human Poly ADP ribose polymerase microplate Human Poly ADP ribose polymerase standard Human Poly ADP ribose polymerase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Poly ADP ribose polymerase (PARP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PARP1. No significant cross-reactivity or interference between Human PARP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PARP is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress . This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo . In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) . PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.
Notes	This Human Poly ADP ribose polymerase (PARP) ELISA Kit employs a two-site sandwich ELISA to quantitate PARP1 in samples. An antibody specific for PARP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	142

Applications	ELISA
Applications Notes	This Human Poly ADP ribose polymerase (PARP) ELISA Kit employs a two-site sandwich ELISA to quantitate PARP1 in samples. An antibody specific for PARP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703518	KTE61302	48 T	$359		Add to Cart
BHE13703518	KTE61302	96 T	$619		Add to Cart
BHE13703518	KTE61302	96 Tx5	$2809		Add to Cart
BHE13703518	KTE61302	96 Tx50	$26209		Add to Cart

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