Matrices listed below were spiked with certain level of PNLIP and the recovery rates were calculated by comparing the measured value to the expected amount of PNLIP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-100
94
EDTA plasma(n=5)
87-105
97
heparin plasma(n=5)
97-104
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PNLIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PNLIP ELISA Kit allows for the in vitro quantitative determination of PNLIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PNLIP(Pancreatic triacylglycerol lipase) ELISA Kit