Human Plexin B2/PLXNB2 ELISA Kit PicoKine®

Mfr.No.

EK1826

Sensitivity

<0.1 ng/ml

Detection Range

0.624 ng/ml - 40 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.63	1.25	2.5	5	10	20	40
O.D.	0.029	0.150	0.299	0.381	0.731	0.929	1.346	1.963

Description

Human Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLXNB2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin-B2; MM1; PLXNB2; KIAA0315

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-L1838

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Plexin B2

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1214	4275	19603	1233	4407	19741
Standard deviation	63.13	239.4	960.55	82.6	339.3	1539.8
CV (%)	5.2%	5.6%	4.9%	6.7%	7.7%	7.8%

Protein Function

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Background

Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.This gene is mapped to 22q13.33.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1214	1018	1165	1017	1103	87.72	7.9%
Sample 2	4275	4570	4511	4741	4524	166.86	3.6%
Sample 3	19603	18628	19571	18283	19021	578.86	3.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	23654
Antigen	B2/PLXNB2
Gene Name	PLXNB2
UniProt ID	O15031

Applications	ELISA
Research Area	Cell Type Marker,Growth and Development,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001414	EK1826	96 wells/kit	$499		Add to Cart

Human Plexin B2/PLXNB2 ELISA Kit PicoKine®

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