Human Plexin B2/PLXNB2 ELISA Kit PicoKine®

Mfr.No.

EK1826

Sensitivity

<0.1 ng/ml

Detection Range

0.624 ng/ml - 40 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.63	1.25	2.5	5	10	20	40
O.D.	0.029	0.150	0.299	0.381	0.731	0.929	1.346	1.963

Description

Human Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLXNB2 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin-B2; MM1; PLXNB2; KIAA0315

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-L1838

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Plexin B2

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1214	4275	19603	1233	4407	19741
Standard deviation	63.13	239.4	960.55	82.6	339.3	1539.8
CV (%)	5.2%	5.6%	4.9%	6.7%	7.7%	7.8%

Protein Function

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Background

Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.This gene is mapped to 22q13.33.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1214	1018	1165	1017	1103	87.72	7.9%
Sample 2	4275	4570	4511	4741	4524	166.86	3.6%
Sample 3	19603	18628	19571	18283	19021	578.86	3.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	23654
Gene Name	PLXNB2
UniProt ID	O15031

Applications	ELISA
Research Area	Cell Type Marker,Growth and Development,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001414	EK1826	96 wells/kit	$499		Add to Cart

Human Plexin B2/PLXNB2 ELISA Kit PicoKine®

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