Human pleiotrophin,PTN ELISA Kit

Human pleiotrophin,PTN ELISA Kit

Reviews of Human pleiotrophin,PTN ELISA Kit

Rate it now..!!

Rate it now..!!

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Mfr.No.

CSB-E09305h

Sensitivity

0.06 ng/mL

Detection Range

0.24 ng/mL-15 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

pleiotrophin, HARP, HBGF8, HBNF, NEGF1, heparin affin regulatory protein, heparin binding growth factor 8, heparin-binding growth-associated molecule, neurite growth-promoting factor 1, pleiotrophin (heparin binding

Recovery

The recovery of mouse ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	92
	Range %	88-95
1:40	Average %	105
	Range %	100-107
1:80	Average %	93
	Range %	87-97
1:160	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
10	2.427	2.411	2.419	2.250
5	1.988	2.022	2.005	1.836
2.5	1.354	1.379	1.367	1.198
1.25	0.780	0.742	0.761	0.592
0.625	0.495	0.464	0.480	0.311
0.312	0.362	0.377	0.370	0.201
0.156	0.265	0.255	0.260	0.091
0	0.171	0.167	0.169	?

Species

Homo sapiens

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21246
Tissue Specificity	Osteoblast and brain.

Applications	ELISA
Research Area	PTN

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10503824	CSB-E09305h	96T	$835	Add to Cart
BHE10503824	CSB-E09305h	5x96T	$4008	Add to Cart
BHE10503824	CSB-E09305h	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.