Human Platelet-activating Factor Acetylhydrolase 2

Mfr.No.

E5889Hu

Sensitivity

0.221ng/mL

Detection Range

0.5-150ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

HSD-PLA2;PAF:lysophospholipid transacetylase;PAF:sphingosine transacetylase;PAFAH2;Platelet-activating factor acetylhydrolase 2, cytoplasmic;Platelet-activating factor acetyltransferase PAFAH2;SD-PLA2;Serine-dependent phospholipase A2

Molecular Weight

44036

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5889Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	61.3	3.77	6.15
Intra-Assay	2	18	12.39	0.69	5.57
Intra-Assay	3	18	23.32	1.31	5.64

Background

Platelet-activating factor acetylhydrolase 2, cytoplasmic is an enzyme that in humans is encoded by the PAFAH2 gene. It is one of several PAF acetylhydrolases.

Protocol

These standard curves of E5889Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/mL	2.128	2.11	2.046
	2.092
40ng/mL	1.45	1.449	1.385
	1.448
20ng/mL	0.944	0.917	0.853
	0.889
10ng/mL	0.473	0.487	0.422
	0.5
5ng/mL	0.301	0.268	0.204
	0.235
0ng/mL	0.057	0.064	0
	0.071

Gene ID	5051
UniProt ID	Q99487

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107251	E5889Hu	48T	$320		Add to Cart
BHE12107251	E5889Hu	96T	$458		Add to Cart

Human Platelet-activating Factor Acetylhydrolase 2 - PAFAH2 ELISA Kit

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