Human Platelet-activating Factor Acetylhydrolase 2 - Cytoplasmic

Mfr.No.

E2532Hu

Alternative Names

HSD-PLA2;PAF:lysophospholipid transacetylase;PAF:sphingosine transacetylase;PAFAH2;Platelet-activating factor acetylhydrolase 2, cytoplasmic;Platelet-activating factor acetyltransferase PAFAH2;SD-PLA2;Serine-dependent phospholipase A2

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

44036

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/ml

Background

Catalyzes the hydrolyze of the acetyl group at the sn-2 position of platelet-activating factor (PAF) and its analogs, leading to their inactivation (PubMed:9494101). Hydrolyzes propionyl and butyroyl moieties approximately half as effectively as PAF (By similarity). Also catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position (By similarity).

Gene ID	5051
UniProt ID	Q99487
Antigen	PAFAH2

Research Area

Signal Transduction

Protocol

These standard curves of E2532Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.094	1.958	1.915
	1.822
3.2ng/ml	1.056	0.983	0.941
	0.91
1.6ng/ml	0.476	0.467	0.425
	0.459
0.8ng/ml	0.208	0.253	0.211
	0.299
0.4ng/ml	0.132	0.12	0.078
	0.108
0ng/ml	0.029	0.042	0
	0.056

Precision

We measured random samples of E2532Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.16	0.13	2.49
Intra-Assay	2	18	2.95	0.14	4.66
Intra-Assay	3	18	1.36	0.07	5.41

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104070	E2532Hu	48T	$320		Add to Cart
BHE12104070	E2532Hu	96T	$458		Add to Cart

Human Platelet-activating Factor Acetylhydrolase 2 - Cytoplasmic - PAFAH2 ELISA Kit

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