Human Placenta Growth Factor

Mfr.No.

E0138Hu

Description

This sandwich kit is for the accurate quantitative detection of Placenta Growth Factor (also known as PLGF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

PGF;Placenta growth factor;PlGF

Reactivity

Human

Detection Range

8-1800ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

24789

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.02ng/L

Background

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

Gene ID	5228
UniProt ID	P49763
Antigen	PGF

Research Area

Cancer

Protocol

These standard curves of E0138Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.395	2.258	2.216
	2.122
480ng/L	1.359	1.345	1.303
	1.332
240ng/L	0.72	0.732	0.69
	0.744
120ng/L	0.557	0.541	0.499
	0.525
60ng/L	0.239	0.242	0.2
	0.245
0ng/L	0.039	0.042	0
	0.045

Precision

We measured random samples of E0138Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	260.5	16.68	6.4
Intra-Assay	2	18	637.3	23.43	3.7
Intra-Assay	3	18	210.4	16.01	7.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101873	E0138Hu	96T	$458		Add to Cart
BHE12101873	E0138Hu	48T	$320		Add to Cart

Human Placenta Growth Factor - PLGF ELISA Kit

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