Human Pituitary Homeobox 1 - PITX1 ELISA Kit

Mfr.No.

E5877Hu

Alternative Names

Hindlimb-expressed homeobox protein backfoot;Homeobox protein PITX1;Paired-like homeodomain transcription factor 1;Pituitary homeobox 1;PITX 1;PITX1

Reactivity

Human

Detection Range

7-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34128

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.34ng/L

Background

Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.

Gene ID	5307
UniProt ID	P78337
Antigen	PITX1

Research Area

Signal Transduction

Protocol

These standard curves of E5877Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.367	2.253	2.216
	2.138
400ng/L	1.334	1.248	1.211
	1.163
200ng/L	0.714	0.736	0.699
	0.758
100ng/L	0.382	0.382	0.345
	0.383
50ng/L	0.18	0.212	0.175
	0.243
0ng/L	0.038	0.037	0
	0.036

Precision

We measured random samples of E5877Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	302.8	15.02	5
Intra-Assay	2	18	411.7	14.4	3.5
Intra-Assay	3	18	94.2	4.84	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107239	E5877Hu	48T	$320		Add to Cart
BHE12107239	E5877Hu	96T	$458		Add to Cart

Human Pituitary Homeobox 1 - PITX1 ELISA Kit

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