Matrices listed below were spiked with certain level of PIEZO1 and the recovery rates were calculated by comparing the measured value to the expected amount of PIEZO1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
94
EDTA plasma(n=5)
87-101
94
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIEZO1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-102%
85-96%
90-104%
EDTA plasma(n=5)
82-92%
83-99%
88-101%
heparin plasma(n=5)
88-100%
86-100%
85-98%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q92508
Antigen
PIEZO1
Applications
ELISA
Applications Notes
PIEZO1 ELISA Kit allows for the in vitro quantitative determination of PIEZO1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PIEZO1(Piezo-type mechanosensitive ion channel component 1) ELISA Kit