Human Phosphoglycerate Mutase 2

Mfr.No.

E0756Hu

Sensitivity

5.29ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BPG-dependent PGAM 2;Muscle-specific phosphoglycerate mutase;PGAM 2;PGAM2;PGAM-M;Phosphoglycerate mutase 2;Phosphoglycerate mutase isozyme M

Molecular Weight

28766

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0756Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	715.5	45.5	6.4
Intra-Assay	2	18	772.9	27.07	3.5
Intra-Assay	3	18	359.5	12.69	3.5

Background

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Protocol

These standard curves of E0756Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.77	1.721	1.664
	1.672
600ng/L	1.046	1.068	1.011
	1.09
300ng/L	0.558	0.56	0.503
	0.561
150ng/L	0.169	0.195	0.138
	0.22
75ng/L	0.078	0.104	0.048
	0.131
0ng/L	0.051	0.057	0
	0.062

Gene ID	5224
UniProt ID	P15259

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102435	E0756Hu	96T	$458		Add to Cart
BHE12102435	E0756Hu	48T	$320		Add to Cart

Human Phosphoglycerate Mutase 2 - PGAM2 ELISA Kit

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