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Home / ELISA Kit

Human Phosphoglucomutase-2 (PGM2) ELISA Kit

SKU : BHE13703442

The PGM2 protein shares about 20% identity with mammalian PGM1. Sequence analysis suggested that PGM2 is a cytosolic protein. Quantitative RT-PCR of mouse tissues detected the highest expression levels of PGM2 in lung, spleen, and thymus. PGM2 acted more than 10-fold better as a phosphopentomutasethan as a phosphoglucomutase. PGM2 may play a role in congenital immunodeficiencies. phosphopentomutase uses ribose 1-phosphate and deoxyribose 1-phosphate, which are formed by purine nucleoside phosphorylase and uridine phosphorylase, and that the absence of phosphopentomutase should result in the accumulation of ribose 1-phosphate and deoxyribose 1-phosphate and therefore in a functional block of purine nucleoside phosphorylase.

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Mfr.No.	KTE61226
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Phosphoglucomutase-2 (PGM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PGM2.
Alternative Names	PGM2; FLJ10983; MSTP006; glucose phosphomutase 2
Detection Method	Colorimetric
Kit Components	Human Phosphoglucomutase-2 microplate Human Phosphoglucomutase-2 standard Human Phosphoglucomutase-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Phosphoglucomutase-2 (PGM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PGM2. No significant cross-reactivity or interference between Human PGM2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The PGM2 protein shares about 20% identity with mammalian PGM1. Sequence analysis suggested that PGM2 is a cytosolic protein. Quantitative RT-PCR of mouse tissues detected the highest expression levels of PGM2 in lung, spleen, and thymus. PGM2 acted more than 10-fold better as a phosphopentomutasethan as a phosphoglucomutase. PGM2 may play a role in congenital immunodeficiencies. phosphopentomutase uses ribose 1-phosphate and deoxyribose 1-phosphate, which are formed by purine nucleoside phosphorylase and uridine phosphorylase, and that the absence of phosphopentomutase should result in the accumulation of ribose 1-phosphate and deoxyribose 1-phosphate and therefore in a functional block of purine nucleoside phosphorylase.
Notes	This Human Phosphoglucomutase-2 (PGM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PGM2 in samples. An antibody specific for PGM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55276

Applications	ELISA
Applications Notes	This Human Phosphoglucomutase-2 (PGM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PGM2 in samples. An antibody specific for PGM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703442	KTE61226	48 T	$339		Add to Cart
BHE13703442	KTE61226	96 T	$589		Add to Cart
BHE13703442	KTE61226	96 Tx5	$2629		Add to Cart
BHE13703442	KTE61226	96 Tx50	$24569		Add to Cart

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