Human Phosphatidylethanolamine N-methyltransferase

Mfr.No.

E5862Hu

Sensitivity

7.45ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PEAMT;PEMPT;PEMT;PEMT2;Phosphatidylethanolamine N-methyltransferase;Phospholipid methyltransferase;PLMT;PNMT

Molecular Weight

22134

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5862Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	675.67	36.14	5.35
Intra-Assay	2	18	978.88	37.08	3.79
Intra-Assay	3	18	258.41	12.62	4.88

Background

Catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC).

Protocol

These standard curves of E5862Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.23	2.192	2.154
	2.154
800ng/L	1.392	1.351	1.313
	1.311
400ng/L	0.585	0.664	0.626
	0.742
200ng/L	0.318	0.328	0.29
	0.338
100ng/L	0.152	0.152	0.114
	0.152
0ng/L	0.048	0.038	0
	0.028

Gene ID	10400
UniProt ID	Q9UBM1

Research Area	Tags & Cell Markers; Signal Transduction; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107224	E5862Hu	48T	$320		Add to Cart
BHE12107224	E5862Hu	96T	$458		Add to Cart

Human Phosphatidylethanolamine N-methyltransferase - PEMT ELISA Kit

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