Human Phosphatase and Actin Regulator 1

Mfr.No.

E5860Hu

Alternative Names

DEE70;dJ257A7.2;EIEE70;PHACTR1;Phosphatase and actin regulator 1;RPEL;RPEL1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

66308

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.86ng/L

Background

Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity (PubMed:21798305, PubMed:21939755). Involved in the regulation of cortical neuron migration and dendrite arborization (By similarity).

Gene ID	221692
UniProt ID	Q9C0D0
Antigen	PHACTR1

Research Area

Signal Transduction

Protocol

These standard curves of E5860Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.832	1.891	1.864
	1.95
800ng/L	0.971	1.028	1.002
	1.085
400ng/L	0.516	0.542	0.515
	0.567
200ng/L	0.202	0.182	0.155
	0.162
100ng/L	0.066	0.066	0.04
	0.066
0ng/L	0.03	0.027	0
	0.024

Precision

We measured random samples of E5860Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1267.02	38.84	3.07
Intra-Assay	2	18	518.03	27.16	5.24
Intra-Assay	3	18	795.49	40.97	5.15

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107222	E5860Hu	48T	$320		Add to Cart
BHE12107222	E5860Hu	96T	$458		Add to Cart

Human Phosphatase and Actin Regulator 1 - PHACTR1 ELISA Kit

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