Matrices listed below were spiked with certain level of PGK1 and the recovery rates were calculated by comparing the measured value to the expected amount of PGK1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
93
EDTA plasma(n=5)
85-100
92
heparin plasma(n=5)
88-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PGK1 ELISA Kit allows for the in vitro quantitative determination of PGK1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PGK1(PhosphoglyceRate kinase 1) ELISA Kit