Human Peroxisomal Multifunctional Enzyme Type 2

Mfr.No.

E5852Hu

Alternative Names

17-beta-HSD 4;17-beta-hydroxysteroid dehydrogenase 4;3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase;3R-hydroxyacyl-CoA dehydrogenase;D-bifunctional protein;DBP;Enoyl-CoA hydratase 2;HSD 17B4;HSD17B4;MFE-2;MFP-2;Multifunctional protein 2;Peroxisomal multifunctional enzyme type 2;Short chain dehydrogenase/reductase family 8C member 1 (Cleaved into:

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

79686

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.14ng/L

Background

Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from straight-chain, 2-methyl-branched-chain fatty acids bile acid intermediates. With EHHADH, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed: 10671535).

Gene ID	3295
UniProt ID	P51659
Antigen	HSD17B4

Research Area

Others

Protocol

These standard curves of E5852Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.258	2.155	2.109
	2.052
600ng/L	1.413	1.408	1.362
	1.403
300ng/L	0.829	0.794	0.748
	0.759
150ng/L	0.521	0.483	0.437
	0.445
75ng/L	0.274	0.246	0.2
	0.218
0ng/L	0.035	0.046	0
	0.057

Precision

We measured random samples of E5852Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	843.2	35.04	4.2
Intra-Assay	2	18	270	15.65	5.8
Intra-Assay	3	18	225.2	13.83	6.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107214	E5852Hu	48T	$320		Add to Cart
BHE12107214	E5852Hu	96T	$458		Add to Cart

Human Peroxisomal Multifunctional Enzyme Type 2 - HSD17B4 ELISA Kit

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