Human Periostin - POSTN ELISA Kit

Mfr.No.

E3226Hu

Sensitivity

0.251ng/ml

Detection Range

0.5-150ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Periostin (also known as POSTN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

OSF-2;Osteoblast-specific factor 2;Periostin;PN;POSTN

Molecular Weight

93314

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3226Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	47	1.32	2.8
Intra-Assay	2	18	37.3	1.92	5.2
Intra-Assay	3	18	14.7	0.68	4.6

Background

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed: 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

Protocol

These standard curves of E3226Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
96ng/ml	2.327	2.276	2.234
	2.224
48ng/ml	1.456	1.425	1.383
	1.394
24ng/ml	0.757	0.701	0.659
	0.645
12ng/ml	0.313	0.35	0.308
	0.387
6ng/ml	0.122	0.121	0.079
	0.119
0ng/ml	0.037	0.042	0
	0.046

Gene ID	10631
UniProt ID	Q15063

Research Area	Cardiovascular

Storage	2-8°C

Reference	The Predictive Value of Periostin to Diagnose Crohn's Disease Journal: Turk J Gastroenterol. 2022 Feb;33(2):127-135.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104755	E3226Hu	48T	$320		Add to Cart
BHE12104755	E3226Hu	96T	$458		Add to Cart

Human Periostin - POSTN ELISA Kit

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