Human Perilipin-1 - PLIN1 ELISA Kit

Mfr.No.

E3223Hu

Sensitivity

0.043ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Perilipin 1 (also known as PLIN1) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Lipid droplet-associated protein;Perilipin-1;PLIN 1;PLIN1

Molecular Weight

55990

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3223Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.5	0.56	3
Intra-Assay	2	18	12.4	0.61	4.9
Intra-Assay	3	18	5.6	0.26	4.7

Background

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.

Protocol

These standard curves of E3223Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.831	1.952	1.913
	2.073
12ng/ml	0.937	1.033	0.994
	1.129
6ng/ml	0.438	0.507	0.467
	0.575
3ng/ml	0.3	0.237	0.198
	0.174
1.5ng/ml	0.169	0.165	0.126
	0.162
0ng/ml	0.047	0.039	0
	0.031

Gene ID	5346
UniProt ID	O60240

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104752	E3223Hu	48T	$320		Add to Cart
BHE12104752	E3223Hu	96T	$458		Add to Cart

Human Perilipin-1 - PLIN1 ELISA Kit

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