Human PEDF/SerpinF1 ELISA Kit PicoKine®

Mfr.No.

EK0896

Sensitivity

<20 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.006	0.011	0.240	0.408	0.659	1.021	1.410	1.956

Description

Human PEDF/SerpinF1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINF1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Pigment epithelium-derived factor;PEDF;Cell proliferation-inducing gene 35 protein;EPC-1;Serpin F1;SERPINF1;PEDF;PIG35;

Molecular Weight

46312 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-P418

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PEDF

Subcellular Localization

Secreted . Melanosome . Enriched in stage I melanosomes.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	286	780	2612	271	802	2714
Standard deviation	19.16	44.46	10448	19.51	53.73	130.27
CV (%)	6.7%	5.7%	4%	7.2%	6.7%	4.8%

Protein Function

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. .

Background

Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	286	285	271	293	284	13.05	4.6%
Sample 2	780	796	821	805	801	33.62	4.2%
Sample 3	2612	2744	2752	2653	2690	104.92	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5176
Gene Name	SERPINF1
UniProt ID	P36955
Protein Name	Pigment epithelium-derived factor
Tissue Specificity	Retinal pigment epithelial cells and blood plasma. .

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Invasion/Microenvironment,Neurogenesis,Neurology Process,Neuroscience,Sensory System,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001016	EK0896	96 wells/kit	$499		Add to Cart

Human PEDF/SerpinF1 ELISA Kit PicoKine®

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