Human PEAR1 ELISA Kit PicoKine®

Mfr.No.

EK2266

Sensitivity

<150 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	312	625	1,250	2,500	5,000	10,000	20,000
O.D.	0.030	0.065	0.104	0.273	0.472	0.929	1.465	2.069

Description

Human PEAR1 ELISA Kit PicoKine® (96 Tests). Quantitate Human PEAR1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L21-S754

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PEAR1

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	526	1057	7376	503	771	6486
Standard deviation	34.51	37.1	478.7	16.05	60.75	323.65
CV(%)	6.6%	6.5%	6.5%	3.2%	7.9%	5%

Background

PEAR1 is a platelet receptor that signals upon the formation of platelet-platelet contacts independent of platelet activation and secondary to platelet aggregation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	526	341	576	388	458	28.06	6.1%
Sample 2	1057	774	955	1025	953	55.93	5.9%
Sample 3	7376	9829	5207	8940	7838	581.58	7.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	375033
Gene Name	PEAR1
UniProt ID	Q5VY43

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001815	EK2266	96 wells/kit	$499		Add to Cart

Human PEAR1 ELISA Kit PicoKine®

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