Human Pdz and Lim Domain Protein 1

Mfr.No.

E5835Hu

Alternative Names

C-terminal LIM domain protein 1;Elfin;LIM domain protein CLP-36;PDLIM 1;PDLIM1;PDZ and LIM domain protein 1

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36072

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.62ng/L

Background

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed: 10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

Gene ID	9124
UniProt ID	O00151
Antigen	PDLIM1

Research Area

Others

Protocol

These standard curves of E5835Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.263	2.229	2.193
	2.195
1600ng/L	1.712	1.733	1.697
	1.754
800ng/L	0.958	0.976	0.94
	0.993
400ng/L	0.659	0.709	0.673
	0.759
200ng/L	0.413	0.428	0.392
	0.443
0ng/L	0.038	0.036	0
	0.034

Precision

We measured random samples of E5835Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1200.3	66.25	5.5
Intra-Assay	2	18	667.5	40.33	6
Intra-Assay	3	18	385.1	26.07	6.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107197	E5835Hu	48T	$320		Add to Cart
BHE12107197	E5835Hu	96T	$458		Add to Cart

Human Pdz and Lim Domain Protein 1 - PDLIM1 ELISA Kit

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