Matrices listed below were spiked with certain level of PDK4 and the recovery rates were calculated by comparing the measured value to the expected amount of PDK4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-105
98
EDTA plasma(n=5)
91-103
98
heparin plasma(n=5)
85-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDK4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PDK4 ELISA Kit allows for the in vitro quantitative determination of PDK4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit