Human PD-1 ELISA Kit PicoKine®

Mfr.No.

EK0959

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.002	0.054	0.102	0.189	0.360	0.660	1.042	1.907

Description

Human PD-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human PDCD1 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Programmed cell death protein 1;Protein PD-1;hPD-1;CD279;PDCD1;PD1;

Molecular Weight

31647 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L25-Q167

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PD-1

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	17	88	267	22	96	314
Standard deviation	0.71	4.49	13.88	1.12	4.99	19.78
CV (%)	4.2%	5.1%	5.2%	5.1%	5.2%	6.3%

Protein Function

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. .

Background

Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	17	25	30	19	23	0.41	1.8%
Sample 2	88	94	101	79	91	2.35	2.6%
Sample 3	267	294	315	302	295	18.26	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5133
Gene Name	PDCD1
UniProt ID	Q15116
Protein Name	Programmed cell death protein 1

Applications	ELISA
Research Area	Apoptosis,Associated Proteins,Cell Biology,Developmental Biology,Intracellular,Neurogenesis,Neurology Process,Neuroscience,Organogenesis,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000725	EK0959	96 wells/kit	$499		Add to Cart

Human PD-1 ELISA Kit PicoKine®

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