Human PCK2 ELISA Kit

Mfr.No.

E5866Hu

Sensitivity

7.02ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PCK2;PEPCK;PEPCK2;PEPCK-M;Phosphoenolpyruvate carboxykinase (GTP), mitochondrial

Molecular Weight

70699

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5866Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1018.37	35.7	3.51
Intra-Assay	2	18	632.17	31.45	4.97
Intra-Assay	3	18	252.82	16.04	6.35

Background

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Protocol

These standard curves of E5866Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.116	2.104	2.048
	2.092
800ng/L	1.27	1.205	1.149
	1.141
400ng/L	0.784	0.804	0.748
	0.823
200ng/L	0.531	0.471	0.415
	0.412
100ng/L	0.189	0.216	0.16
	0.242
0ng/L	0.053	0.056	0
	0.059

Gene ID	5106
UniProt ID	Q16822

Research Area	Cardiovascular; Signal Transduction; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107228	E5866Hu	48T	$320		Add to Cart
BHE12107228	E5866Hu	96T	$458		Add to Cart

Human PCK2 ELISA Kit

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