Human Pappalysin-2 - PAPPA2 ELISA Kit

Mfr.No.

E5833Hu

Sensitivity

0.215ng/mL

Detection Range

0.5-100ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PAPPA 2;PAPPA2;PAPP-A2;Pappalysin-2;PAPP-E;Pregnancy-associated plasma protein A2;Pregnancy-associated plasma protein E1

Molecular Weight

198539

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5833Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	54.4	1.96	3.6
Intra-Assay	2	18	15.2	0.67	4.4
Intra-Assay	3	18	31.4	1.47	4.7

Background

Metalloproteinase which specifically cleaves Insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3.

Protocol

These standard curves of E5833Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/mL	1.991	2.025	1.978
	2.058
32ng/mL	1.154	1.088	1.041
	1.022
16ng/mL	0.519	0.553	0.506
	0.587
8ng/mL	0.166	0.204	0.158
	0.243
4ng/mL	0.071	0.11	0.064
	0.15
0ng/mL	0.055	0.047	0
	0.038

Gene ID	60676
UniProt ID	Q9BXP8

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107195	E5833Hu	48T	$320		Add to Cart
BHE12107195	E5833Hu	96T	$458		Add to Cart

Human Pappalysin-2 - PAPPA2 ELISA Kit

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