Human p53/tumor protein,p53/TP53 ELISA Kit

Human p53/tumor protein,p53/TP53 ELISA Kit

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Mfr.No.

CSB-E08334h

Sensitivity

2.34 pg/mL

Detection Range

9.38 pg/mL-600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

tumor protein p53, FLJ92943, LFS1, TRP53, p53, p53 antigen, p53 transformation suppressor, p53 tumor suppressor, phosphoprotein p53, transformation-related protein 53

Recovery

The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-97
EDTA plasma (n=4)	94	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	96
	Range %	91-103
1:4	Average %	94
	Range %	90-99
1:8	Average %	94
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	Corrected
1000	2.608	2.555	2.582	2.392
500	1.939	1.897	1.918	1.728
250	1.227	1.212	1.220	1.030
125	0.821	0.759	0.790	0.600
62.5	0.468	0.450	0.459	0.269
31.2	0.331	0.328	0.330	0.140
15.6	0.278	0.271	0.275	0.085
0	0.192	0.188	0.190	?

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Urine, Cerebrospinal fluid

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04637
Tissue Specificity	Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3

Applications	ELISA
Research Area	TP53

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503670	CSB-E08334h	96T	$695	Add to Cart
BHE10503670	CSB-E08334h	5x96T	$3336	Add to Cart
BHE10503670	CSB-E08334h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.