Human P-Cadherin-3 CDH3 ELISA Kit PicoKine®

Mfr.No.

EK0667

Sensitivity

<20 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.192	0.214	0.245	0.305	0.448	0.678	1.217	2.019

Description

Human P-Cadherin-3 CDH3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CDH3 in cell culture supernatants and serum. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cadherin-3;Placental cadherin;P-cadherin;CDH3;CDHP;

Molecular Weight

91418 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D108-G654

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human P-Cadherin

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum

Activating Reagent

Aliquot 0.1 ml per well of the 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml human P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well). Add 0.1 ml of each properly diluted sample of human cell culture supernatants or serum to each empty well. See Sample Dilution Guideline above for details. It is recommended that each human P-Cadherin standard solution and each sample be measured in duplicate.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	262	1066	3298	307	816	3355
Standard deviation	18.24	69.93	148.74	21.18	18.28	100.31
CV(%)	7%	6.6%	4.5%	6.9%	2.2%	3%

Protein Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Background

Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	262	177	211	243	223	7.28	3.3%
Sample 2	1066	781	868	1158	968	63.71	6.6%
Sample 3	3298	3822	3030	2921	3268	184.95	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1001
Gene Name	CDH3
UniProt ID	P22223
Protein Name	Cadherin-3
Tissue Specificity	Expressed in some normal epithelial tissues and in some carcinoma cell lines. .
Sequence	Contains 5 cadherin domains.

Applications	ELISA
Research Area	Cadherins,Cell Adhesion,Cytoskeleton/ECM,Neuroscience,Sensory System,Signal Transduction,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000554	EK0667	96 wells/kit	$499		Add to Cart

Human P-Cadherin-3 CDH3 ELISA Kit PicoKine®

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