Human Oxysterol-binding Protein-related Protein 3

Mfr.No.

E3359Hu

Description

This sandwich kit is for the accurate quantitative detection of human Oxysterol-binding Protein-related Protein 3 (also known as OSBPL3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ORP-3;OSBPL 3;OSBPL3;OSBP-related protein 3;Oxysterol-binding protein-related protein 3

Reactivity

Human

Detection Range

0.1-38ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

101224

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.053ng/ml

Background

Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed: 16143324). Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed: 25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed: 18270267, PubMed: 25447204). With VAPA, may regulate ER morphology (PubMed: 16143324). Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed: 18270267). Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed: 16143324). Also binds 25-hydroxycholesterol and cholesterol (PubMed: 17428193).

Gene ID	26031
UniProt ID	Q9H4L5
Antigen	OSBPL3

Research Area

Others

Protocol

These standard curves of E3359Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	1.687	1.742	1.694
	1.798
10ng/ml	1.062	0.985	0.936
	0.908
5ng/ml	0.566	0.584	0.536
	0.602
2.5ng/ml	0.201	0.202	0.153
	0.202
1.25ng/ml	0.103	0.098	0.049
	0.093
0ng/ml	0.049	0.049	0
	0.049

Precision

We measured random samples of E3359Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.3	0.36	3.9
Intra-Assay	2	18	16.6	0.52	3.1
Intra-Assay	3	18	4.5	0.2	4.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104888	E3359Hu	48T	$320		Add to Cart
BHE12104888	E3359Hu	96T	$458		Add to Cart

Human Oxysterol-binding Protein-related Protein 3 - OSBPL3 ELISA Kit

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