Human Oxysterol Binding Protein Like Protein 8

Mfr.No.

E3358Hu

Sensitivity

0.13ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ORP-8;OSBPL 8;OSBPL8;OSBP-related protein 8;Oxysterol-binding protein-related protein 8

Molecular Weight

101196

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3358Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.1	0.25	3.1
Intra-Assay	2	18	30.3	0.97	3.2
Intra-Assay	3	18	14.6	0.55	3.7

Background

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed: 26206935). Binds oxysterol, 25-hydroxycholesterol and cholesterol (PubMed: 17428193, PubMed: 17991739, PubMed: 21698267).

Protocol

These standard curves of E3358Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
36ng/ml	1.958	1.921	1.876
	1.883
18ng/ml	1.01	1.002	0.957
	0.993
9ng/ml	0.536	0.464	0.419
	0.392
4.5ng/ml	0.179	0.204	0.159
	0.228
2.25ng/ml	0.169	0.122	0.077
	0.076
0ng/ml	0.039	0.045	0
	0.051

Gene ID	114882
UniProt ID	Q9BZF1

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104887	E3358Hu	48T	$320		Add to Cart
BHE12104887	E3358Hu	96T	$458		Add to Cart

Human Oxysterol Binding Protein Like Protein 8 - OSBPL8 ELISA Kit

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