Human Outer Dense Fiber Protein 1

Mfr.No.

E4666Hu

Description

This sandwich kit is for the accurate quantitative detection of human Outer Dense Fiber Protein 1 (also known as ODF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Heat shock protein beta-10;HspB10;ODF 1;ODF1;Outer dense fiber protein 1

Reactivity

Human

Detection Range

20-4000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28366

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.69ng/L

Background

Component of the outer dense fibers (ODF) of spermatozoa. ODF are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail.

Gene ID	4956
UniProt ID	Q14990
Antigen	ODF1

Research Area

Others

Protocol

These standard curves of E4666Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.248	2.162	2.108
	2.075
1000ng/L	1.596	1.606	1.552
	1.615
500ng/L	1.182	1.124	1.07
	1.065
250ng/L	0.639	0.646	0.592
	0.652
125ng/L	0.418	0.447	0.393
	0.475
0ng/L	0.043	0.054	0
	0.065

Precision

We measured random samples of E4666Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1124.7	67.19	6
Intra-Assay	2	18	336.1	20.15	6
Intra-Assay	3	18	219.9	15.88	7.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105962	E4666Hu	48T	$320		Add to Cart
BHE12105962	E4666Hu	96T	$458		Add to Cart

Human Outer Dense Fiber Protein 1 - ODF1 ELISA Kit

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