Matrices listed below were spiked with certain level of OTC and the recovery rates were calculated by comparing the measured value to the expected amount of OTC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-100
95
EDTA plasma(n=5)
86-104
97
heparin plasma(n=5)
98-100
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OTC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
OTC ELISA Kit allows for the in vitro quantitative determination of OTC concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human OTC (Ornithine carbamoyltransferase, mitochondrial) ELISA Kit