Human OPN / Osteopontin ELISA Kit PicoKine®

Mfr.No.

EK0482

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.056	0.110	0.156	0.244	0.457	0.946	1.677	2.085

Description

Human OPN / Osteopontin ELISA Kit PicoKine® (96 Tests). Quantitate Human SPP1 in cell culture supeatants, serum, plasma (heparin, EDTA) , human milk and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin;SPP1;BNSP, OPN;PSEC0156;

Molecular Weight

35423 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: I17-N300

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human OPN

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Human milk, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	305	1771	4985	314	1611	5408
Standard deviation	14.03	138.13	304.08	16.64	133.71	383.96
CV (%)	4.6%	7.8%	6.1%	5.3%	8.3%	7.1%

Protein Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Background

Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	305	309	272	322	302	18.42	6%
Sample 2	1771	1751	1709	1739	1742	22.46	1.2%
Sample 3	4985	4178	4476	4405	4511	294.96	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6696
Gene Name	SPP1
UniProt ID	P10451
Protein Name	Osteopontin
Tissue Specificity	Bone. Found in plasma.
Sequence	Belongs to the osteopontin family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Cytoskeleton/ECM,Extracellular Matrix,Mesenchymal Stem Cells,Signal Transduction,Stem Cells,Structures,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000472	EK0482	96 wells/kit	$499		Add to Cart

Human OPN / Osteopontin ELISA Kit PicoKine®

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