Human Nucleolin - NCL ELISA Kit

Mfr.No.

E5801Hu

Alternative Names

NCL;Nucleolin;Protein C23

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

76614

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.44ng/L

Background

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Gene ID	4691
UniProt ID	P19338
Antigen	NCL

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E5801Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.302	2.363	2.325
	2.423
1200ng/L	1.367	1.408	1.37
	1.45
600ng/L	0.77	0.769	0.731
	0.768
300ng/L	0.376	0.4	0.362
	0.424
150ng/L	0.149	0.168	0.13
	0.187
0ng/L	0.034	0.038	0
	0.042

Precision

We measured random samples of E5801Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	550.5	34.27	6.2
Intra-Assay	2	18	852.6	37.62	4.4
Intra-Assay	3	18	1601.9	50.11	3.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107163	E5801Hu	48T	$320		Add to Cart
BHE12107163	E5801Hu	96T	$458		Add to Cart

Human Nucleolin - NCL ELISA Kit

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