Human NT-proANP/NPPA ELISA Kit PicoKine®

Mfr.No.

EK1627

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.094	0.158	0.228	0.376	0.669	1.101	1.812	2.014

Description

Human NT-proANP/NPPA ELISA Kit PicoKine® (96 Tests). Quantitate Human NPPA in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Natriuretic peptides A; CDD-ANF; Cardiodilatin; CDD; Cardiodilatin-related peptide; CDP; Prepronatriodilatin; Atrial natriuretic factor; ANF; Atrial natriuretic peptide; ANP; NPPA; ANP; PND

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N26-R123

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human NPPA

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	90	200	925	91	182	898
Standard deviation	4.23	13.8	52.72	5.46	15.83	66.45
CV (%)	4.7%	6.9%	5.7%	6%	8.7%	7.4%

Protein Function

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

Background

Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney that is, aldosterone stimulates sodium retention and it generates sodium loss.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	90	94	86	82	88	4.47	5%
Sample 2	200	217	235	206	214	13.31	6.2%
Sample 3	925	929	1045	879	944	61.25	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	878
Gene Name	NPPA
UniProt ID	P01160

Applications	ELISA
Research Area	Cardiovascular

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001334	EK1627	96 wells/kit	$499		Add to Cart

Human NT-proANP/NPPA ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human NT-proANP/NPPA ELISA Kit PicoKine®

Rate it now..!!