Human NT-4/NTF4/Neurotrophin-4 ELISA Kit PicoKine®

Mfr.No.

EK0475

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.021	0.072	0.117	0.195	0.362	0.702	1.288	2.097

Description

Human NT-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human NTF4 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neurotrophin-4;NT-4;Neurotrophin-5;NT-5;Neutrophic factor 4;NTF4;NTF5;

Molecular Weight

22427 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G81-A210

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human NT-4

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	94	234	888	93	224	873
Standard deviation	6.01	13.33	69.26	7.8%5	15.45	84.68
CV (%)	6.4%	5.7%	7.8%	6.4%	6.9%	9.7%

Protein Function

Target-derived survival factor for peripheral sensory sympathetic neurons.

Background

Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons (2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of leaing and memory.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	94	99	93	89	93	3.56	3.8%
Sample 2	234	212	207	213	216	10.35	4.7%
Sample 3	888	924	870	895	894	19.44	2.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4909
Gene Name	NTF4
UniProt ID	P34130
Protein Name	Neurotrophin-4
Tissue Specificity	Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

Applications	ELISA
Research Area	Growth and Development,Neurology Process,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000468	EK0475	96 wells/kit	$499		Add to Cart

Human NT-4/NTF4/Neurotrophin-4 ELISA Kit PicoKine®

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