Human Non-Histone Chromosomal Protein Hmg-14

Mfr.No.

E3182Hu

Sensitivity

5.13ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

High mobility group nucleosome-binding domain-containing protein 1;HMG14;Non-histone chromosomal protein HMG-14

Molecular Weight

10659

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3182Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	318.49	18.21	5.72
Intra-Assay	2	18	747.41	21.75	2.91
Intra-Assay	3	18	566.66	30.48	5.38

Background

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).

Protocol

These standard curves of E3182Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.241	2.175	2.145
	2.109
600ng/L	1.259	1.252	1.223
	1.245
300ng/L	0.647	0.632	0.602
	0.617
150ng/L	0.317	0.342	0.312
	0.368
75ng/L	0.128	0.161	0.131
	0.194
0ng/L	0.04	0.03	0
	0.019

Gene ID	3150
UniProt ID	P05114

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104711	E3182Hu	48T	$320		Add to Cart
BHE12104711	E3182Hu	96T	$458		Add to Cart

Human Non-Histone Chromosomal Protein Hmg-14 - HMG14 ELISA Kit

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