Human Noggin - NOG ELISA Kit

Mfr.No.

E1494Hu

Sensitivity

0.27ng/mL

Detection Range

0.5-200ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Noggin (also known as NOG) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

NOG;Noggin

Molecular Weight

25774

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1494Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	22.9	1.07	4.7
Intra-Assay	2	18	38.4	1.7	4.4
Intra-Assay	3	18	85.6	3.71	4.3

Background

Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed: 21976273, PubMed: 26643732).

Protocol

These standard curves of E1494Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/mL	1.983	2.069	2.031
	2.156
60ng/mL	1.526	1.448	1.41
	1.37
30ng/mL	0.553	0.658	0.619
	0.762
15ng/mL	0.326	0.393	0.355
	0.46
7.5ng/mL	0.158	0.161	0.122
	0.163
0ng/mL	0.031	0.038	0
	0.045

Gene ID	9241
UniProt ID	Q13253

Research Area	Developmental Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103110	E1494Hu	48T	$320		Add to Cart
BHE12103110	E1494Hu	96T	$458		Add to Cart

Human Noggin - NOG ELISA Kit

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