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Home / ELISA Kit

Human NKG2D ligand 2 (ULBP2) ELISA Kit

SKU : BHE13702308

Human cytomegalovirus (CMV) frequently infects young people and persists for the lifetime of the individual in a latent state, due in part to downregulation of major histocompatibility (MHC) class I expression and evasion of T cell-mediated immunity, with occasional asymptomatic episodes of viral shedding.
Instead of a transmembrane domain, ULBP3 has a putative glycosylphosphatidylinositol (GPI) linkage to the membrane. Enzymatic analysis confirmed that the ULBPs are indeed GPI-linked at the cell surface. RT-PCR analysis detected ULBP expression in T, B, and erythroleukemia cell lines and in a wide range of tissues. Expression was upregulated in colon and stomach tumors and downregulated in kidney tumors. FACS analysis demonstrated that protein expression generally correlated with message expression.

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Mfr.No.	KTE60089
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human NKG2D ligand 2 (ULBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ULBP2.
Alternative Names	ULBP2; N2DL2; RAET1H; ALCAN-alpha; NKG2D ligand 2; UL16-binding protein 2; retinoic acid early transcript 1 H
Detection Method	Colorimetric
Kit Components	Human NKG2D ligand 2 microplate Human NKG2D ligand 2 standard Human NKG2D ligand 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human NKG2D ligand 2 (ULBP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ULBP2. No significant cross-reactivity or interference between Human ULBP2 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Human cytomegalovirus (CMV) frequently infects young people and persists for the lifetime of the individual in a latent state, due in part to downregulation of major histocompatibility (MHC) class I expression and evasion of T cell-mediated immunity, with occasional asymptomatic episodes of viral shedding. Instead of a transmembrane domain, ULBP3 has a putative glycosylphosphatidylinositol (GPI) linkage to the membrane. Enzymatic analysis confirmed that the ULBPs are indeed GPI-linked at the cell surface. RT-PCR analysis detected ULBP expression in T, B, and erythroleukemia cell lines and in a wide range of tissues. Expression was upregulated in colon and stomach tumors and downregulated in kidney tumors. FACS analysis demonstrated that protein expression generally correlated with message expression.
Notes	This Human NKG2D ligand 2 (ULBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ULBP2 in samples. An antibody specific for ULBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyULBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ULBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ULBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	80328

Applications	ELISA
Applications Notes	This Human NKG2D ligand 2 (ULBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ULBP2 in samples. An antibody specific for ULBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyULBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ULBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ULBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702308	KTE60089	48 T	$339		Add to Cart
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BHE13702308	KTE60089	96 Tx50	$24569		Add to Cart

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