Human Nitric Oxide Synthase - Brain

Mfr.No.

E0924Hu

Alternative Names

bNOS;Constitutive NOS;IHPS1;NC-NOS;Neuronal NOS;Nitric oxide Synthase, brain;nNOS;N-NOS;NOS;NOS type I;Nos1;Peptidyl-cysteine S-nitrosylase NOS1

Reactivity

Human

Detection Range

0.1-40ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

160970

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.053ng/ml

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Gene ID	4842
UniProt ID	P29475
Antigen	Nos1

Research Area

Neuroscience

Protocol

These standard curves of E0924Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.762	1.77	1.735
	1.778
12ng/ml	1.068	1.042	1.007
	1.016
6ng/ml	0.551	0.518	0.483
	0.485
3ng/ml	0.165	0.223	0.188
	0.28
1.5ng/ml	0.105	0.108	0.073
	0.111
0ng/ml	0.032	0.035	0
	0.038

Precision

We measured random samples of E0924Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.2	0.25	3.96
Intra-Assay	2	18	16.33	0.52	3.16
Intra-Assay	3	18	11.92	0.58	4.83

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102599	E0924Hu	48T	$320		Add to Cart
BHE12102599	E0924Hu	96T	$458		Add to Cart

Human Nitric Oxide Synthase - Brain - NOS1 ELISA Kit

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