Human Nidogen-1 / Entactin / NID-1 / NID1 ELISA Kit PicoKine®

Mfr.No.

EK0856

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.068	0.154	0.217	0.343	0.575	1.063	1.696	2.048

Description

Human Nidogen-1 / Entactin / NID-1 / NID1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NID1 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Nidogen-1;NID-1;Entactin;NID1;NID;

Molecular Weight

136377 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-K1114

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Nidogen-1

Subcellular Localization

Secreted, extracellular space, extracellular matrix, basement membrane.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	132	391	1350	121	413	1428
Standard deviation	5.41	17.98	76.95	6.65	26.43	108.52
CV (%)	4.1%	4.6%	5.7%	5.5%	6.4%	7.6%

Protein Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions.

Background

Nidogen-1 (NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans (heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it (along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	132	145	147	147	142	6.25	4.4%
Sample 2	391	380	383	427	395	18.76	4.7%
Sample 3	1350	1536	1504	1575	1491	85.33	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4811
Gene Name	NID1
UniProt ID	P14543
Protein Name	Nidogen-1
Sequence	Contains 6 EGF-like domains.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000661	EK0856	96 wells/kit	$499		Add to Cart

Human Nidogen-1 / Entactin / NID-1 / NID1 ELISA Kit PicoKine®

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