Human Nicotinamide N-methyltransferase

Mfr.No.

E3440Hu

Sensitivity

9.06ng/L

Detection Range

20-3800ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Nicotinamide N-methyltransferase (also known as NNMT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Nicotinamide N-methyltransferase;NNMT

Molecular Weight

29574

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3440Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	588.8	26.54	4.5
Intra-Assay	2	18	1450.9	48.29	3.3
Intra-Assay	3	18	439.4	23.93	5.4

Background

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Protocol

These standard curves of E3440Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.099	2.152	2.111
	2.204
1000ng/L	1.3	1.343	1.302
	1.385
500ng/L	0.771	0.68	0.639
	0.59
250ng/L	0.4	0.384	0.343
	0.369
125ng/L	0.146	0.16	0.119
	0.174
0ng/L	0.045	0.041	0
	0.037

Gene ID	4837
UniProt ID	P40261

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104962	E3440Hu	48T	$320		Add to Cart
BHE12104962	E3440Hu	96T	$458		Add to Cart

Human Nicotinamide N-methyltransferase - NNMT ELISA Kit

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