Human Neutrophil Cytosolic Factor 2

Mfr.No.

E3916Hu

Sensitivity

2.59ng/L

Detection Range

5-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Neutrophil Cytosolic Factor 2 (also known as NCF2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

67 kDa neutrophil oxidase factor;NADPH oxidase activator 2;NCF 2;NCF2;NCF-2;Neutrophil cytosol factor 2;Neutrophil NADPH oxidase factor 2;p67-phox

Molecular Weight

59762

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3916Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	98.5	5.9	6
Intra-Assay	2	18	288	14.56	5.1
Intra-Assay	3	18	160.4	7.74	4.8

Background

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Protocol

These standard curves of E3916Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.07	2.11	2.055
	2.15
400ng/L	1.716	1.729	1.674
	1.743
200ng/L	1.273	1.223	1.168
	1.173
100ng/L	0.656	0.636	0.581
	0.616
50ng/L	0.429	0.409	0.354
	0.389
0ng/L	0.049	0.055	0
	0.062

Gene ID	4688
UniProt ID	P19878

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105354	E3916Hu	48T	$320		Add to Cart
BHE12105354	E3916Hu	96T	$458		Add to Cart

Human Neutrophil Cytosolic Factor 2 - NCF2 ELISA Kit

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