Human Neuron-specific enolase,NSE ELISA Kit

Human Neuron-specific enolase,NSE ELISA Kit

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Mfr.No.

CSB-E07961h

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

enolase 2 (gamma, neuronal), NSE, 2-phospho-D-glycerate hydrolyase, enolase 2, neural enolase, neuron specific gamma enolase, neurone-specific enolase

Recovery

The recovery of canine AChRab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	92	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine AChRab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-102
1:2	Average %	95
	Range %	84-100
1:4	Average %	96
	Range %	90-103
1:8	Average %	94
	Range %	82-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
240	2.954	2.918	2.936	2.832
120	2.512	2.631	2.572	2.468
60	1.852	1.873	1.863	1.759
30	1.034	0.996	1.015	0.911
15	0.472	0.494	0.483	0.379
7.5	0.261	0.276	0.269	0.165
3.75	0.155	0.161	0.158	0.054
0	0.102	0.106	0.104	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P09104
Tissue Specificity	The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Applications	ELISA
Research Area	ENO2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503548	CSB-E07961h	96T	$595	Add to Cart
BHE10503548	CSB-E07961h	5x96T	$2856	Add to Cart
BHE10503548	CSB-E07961h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.