Human Neurogenic Locus Notch Homolog Protein 4

Mfr.No.

E3264Hu

Sensitivity

14.71ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Neurogenic Locus Notch Homolog Protein 4 (also known as NOTCH4) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

hNotch4;INT3;Neurogenic locus notch homolog protein 4;Notch 4;Notch 4 extracellular truncation;Notch 4 intracellular domain);NOTCH4

Molecular Weight

209622

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3264Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	745.54	45.88	6.15
Intra-Assay	2	18	3453.61	129.67	3.75
Intra-Assay	3	18	1794.06	85.57	4.77

Background

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).

Protocol

These standard curves of E3264Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.408	2.348	2.298
	2.289
2400ng/L	1.335	1.382	1.332
	1.428
1200ng/L	0.729	0.7	0.65
	0.671
600ng/L	0.464	0.474	0.424
	0.483
300ng/L	0.259	0.256	0.206
	0.252
0ng/L	0.051	0.05	0
	0.049

Gene ID	4855
UniProt ID	Q99466

Research Area	Others

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12104793	E3264Hu	48T	$320		Add to Cart
BHE12104793	E3264Hu	96T	$458		Add to Cart

Human Neurogenic Locus Notch Homolog Protein 4 - NOTCH4 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Neurogenic Locus Notch Homolog Protein 4 - NOTCH4 ELISA Kit

Rate it now..!!