Human Neurofilament - Light Polypeptide

Mfr.No.

E4645Hu

Sensitivity

0.049ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

68 kDa neurofilament protein;NEFL;Neurofilament light polypeptide;Neurofilament triplet L protein;NF-L

Molecular Weight

61517

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4645Hu within the same batch/lot to ensure the consistency of the kits' performances.

Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Protocol

These standard curves of E4645Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.095	2.236	2.19
	2.377
10ng/ml	1.561	1.652	1.606
	1.743
5ng/ml	1.153	1.099	1.053
	1.045
2.5ng/ml	0.673	0.712	0.666
	0.752
1.25ng/ml	0.47	0.453	0.408
	0.436
0ng/ml	0.047	0.046	0
	0.045

Gene ID	4747
UniProt ID	P07196

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105947	E4645Hu	48T	$320		Add to Cart
BHE12105947	E4645Hu	96T	$458		Add to Cart

Human Neurofilament - Light Polypeptide - NEFL ELISA Kit

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