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Home / ELISA Kit

Human Neurocalcin-delta (NCALD) ELISA Kit

SKU : BHE13703566

NCALD encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. Neurocalcin-delta contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. Neurocalcin-delta is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. Neurocalcin-delta is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of NCALD have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.

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Mfr.No.	KTE61351
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Neurocalcin-delta (NCALD) ELISA Kit employs a two-site sandwich ELISA to quantitate NCALD.
Alternative Names	NCALD; MGC33870; MGC74858;
Detection Method	Colorimetric
Kit Components	Human Neurocalcin-delta microplate Human Neurocalcin-delta standard Human Neurocalcin-delta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Neurocalcin-delta (NCALD) ELISA Kit has high sensitivity and excellent specificity for detection of Human NCALD. No significant cross-reactivity or interference between Human NCALD and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	NCALD encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. Neurocalcin-delta contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. Neurocalcin-delta is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. Neurocalcin-delta is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of NCALD have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
Notes	This Human Neurocalcin-delta (NCALD) ELISA Kit employs a two-site sandwich ELISA to quantitate NCALD in samples. An antibody specific for NCALD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCALD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCALD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCALD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	83988

Applications	ELISA
Applications Notes	This Human Neurocalcin-delta (NCALD) ELISA Kit employs a two-site sandwich ELISA to quantitate NCALD in samples. An antibody specific for NCALD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCALD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCALD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCALD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703566	KTE61351	48 T	$339		Add to Cart
BHE13703566	KTE61351	96 T	$589		Add to Cart
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BHE13703566	KTE61351	96 Tx50	$24569		Add to Cart

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