Human Neudesin ELISA Kit PicoKine®

Mfr.No.

EK1946

Sensitivity

<15 pg/ml

Detection Range

93.6 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.6	187	375	750	1500	3000	6000
O.D.	0.058	0.119	0.181	0.257	0.482	0.910	1.522	2.263

Description

Human Neudesin ELISA Kit PicoKine® (96 Tests). Quantitate Human NENF in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neudesin; Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47; Secreted protein of unknown function; SPUF protein; NENF; CIR2; SPUF

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G32-F172

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Neudesin

Subcellular Localization

Extracellular space

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	124	715	2078	159	739	2238
Standard deviation	7.69	32.18	118.45	7.79	42.86	140.99
CV (%)	6.2%	4.5%	5.7%	4.9%	5.8%	6.3%

Protein Function

Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance. Plays a role in the human tumorigenesis.

Background

This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alteate splicing of this gene results in multiple transcript variants. It is mapped to 1q32.3.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	124	126	128	129	126	1.92	1.5%
Sample 2	715	739	809	714	744	38.69	5.2%
Sample 3	2078	2149	2030	2027	2071	49.37	2.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29937
Gene Name	NENF
UniProt ID	Q9UMX5
Tissue Specificity	Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.

Applications	ELISA
Research Area	Cell Type Markers,Growth and Development,Neurogenesis,Neurology Process,Neuroscience,Tags & Cell Markers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001468	EK1946	96 wells/kit	$750		Add to Cart

Human Neudesin ELISA Kit PicoKine®

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